Abstract
Liposome is the one
way of encapsulation of extracts for reducing the extract degradation. This study was to prepare
the liposome entrapped extracts of Artocarpus
lakoocha Roxb. (L-Al) and Glycyrrhiza
glabra(L-Gg) alone and in combination
of A. lakoocha and G. glabra extracts (L-AlGg). The liposomes were
prepared by Mechanochemical method and freeze-drying. For stability of
liposomes, storage at 4, 25 and 45°C for 8 weeks was performed. The trapping efficiency
of liposomes and tyrosinase inhibitory activities of extracts entrapped in
liposome were investigated. Results showed liposome
morphology was the spherical vesicles evaluated by TEM. Before freeze-drying, liposomes had particle sizes of 156.966 ± 0.808, 140.8 ± 0.818 and158.633 ± 4.193 nm for L-Al, L-Gg
and L-AlGg, respectively. The entrapment
efficiency of L-Al,
L-Gg
and L-AlGg
was found to be 95.83 ± 13.48, 97.99 ± 5.23 and93.90 ± 16.28 %, respectively. The tyrosinase inhibitory
activities of released extracts from L-Al, L-Gg and L-AlGg were 81.57 ± 1.22, 68.92 ± 1.23 and81.40 ± 0.64 %, respectively. After freeze-drying, the particle sizes of L-Al and L-AlGg were no
significant changes, while L-Gg particle size was
bigger (p < 0.01). The liposome entrapment and tyrosinase
inhibitory activity of released extracts were not significantly changed after
freeze-drying. This indicates good
stability and no extract leakage of liposomes. In storage at 4°C for 8 weeks, the entrapment efficiency of L-Al, L-Gg and L-AlGg and tyrosinase
inhibitory activity of released extracts were not significantly different,
comparing with controls. When increasing temperature of storage
effected on the significantly reduction of the entrapment of liposomes and the
tyrosinase inhibitory activity of released extracts (p < 0.01). Therefore, the freeze-dried liposome and
storage at low temperature is recommended for stabilizing liposome and extract
quality.
Keywords:Artocarpus lakoocha
Roxb, Glycyrrhiza glabra, Liposome, Stability