Abstract
Leum
Phua Khaow-Mak
is a fermented food, which provideshigh amounts of
nutrients and antioxidant bioactive compounds such as phenolic compounds. Leum
PhuaKhaow-Makextract compounds also have potential
for applications in cosmetics and function food formulations. The objective of this research was to investigate cytotoxic
activity of
crude extracts from Leum Phua Khaow-Makto human dermal fibroblast cells using
sulforhodamine B (SRB) colorimetric
assay method. Leum Phua glutinous
rice (Oryza sativa L. variety Leum Phua) was fermented with a starter (Look Pang) at room temperature for 5 days. Fermented rice samples
were extracted with 95% ethanol for 24 hours.The solvent was evaporated at 45 OC to obtain dried crudeextracts. The antioxidant capacity of crude
extractswas
determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. Butylated hydroxytoluene (BHT) was used as a standard antioxidant.The EC50 value of the extract was 0.367 mg/ml,
as similar to
that of BHT (EC50 = 0.239 mg/ml). A
dried sample was dissolved with 10% (v/v) dimethyl sulfoxide (DMSO) in
the cell culture media. Cytotoxic activity of crude
extracts to human dermal fibroblast cell was evaluated. The
cell cultures were fixed in ice-cold 40% trichloroacetic
acid (TCA) and were dyed using 0.05 % sulforhodamine B for 30 min. Sodium
lauryl sulfate was used as the positive control. The
optical density was measured at 510 nm in a microplate reader. The
percentage of viability of human dermal fibroblast cell were calculated and compared with sodium lauryl
sulfate. The results showed that the concentrations of crude extracts from Leum Phua Khaow-Mak at 0.0001-1.0 mg/ml
have the percentage of viability at 105.17-102.29 respectively. The
percentage of viability of sodium lauryl sulfate at
0.0001-1.0 mg/ml have the percentage viability at 99.43-4.91 respectively. The percentage of viability at
the concentrations of 0.1
and 1.0 mg/ml were low. These obtained results indicated that the
concentrations of crude
extracts from Leum Phua Khaow-Mak in
the range between 0.0001 and 1 mg/ml
were non-toxic to human dermal fibroblast cells.
Keywords: Cytotoxicity,Fibroblast
Cell, Khaow-Mak, Sulforhodamine B
