Abstract
Chenopodium botrys (C. botrys) methanolic extract (Cb.Cr) and subsequent fractions were screened for inhibitory potentials against cholinesterase's. Acetyl-cholinesterase (AChE) and butyryl-cholinesterase (BChE) in vitro inhibitory potentials were evaluated employing Ellman's assay. Lineweaver-Burk a plot (1/v versus 1/[s]) in which v is the velocity of reaction and [s] is substrate concentration was sketched by Michaelis-Menten kinetics. In AChE inhibition assay, chloroform (Cb.Chf), ethyl acetate (Cb.EtAc) and crude extract (Cb.Cr) showed highest activity with 80.12±1.97, 71.79±0.67 and 69.00±1.52% inhibitions at concentration of 1 mg ml-1 with IC50 values of 50, 115 and 130 μg ml-1 respectively. Similarly, Cb.Chf, Cb.EtAc and Cb.Cr showed the strongest activity against BChE causing 76.20±0.28, 70.48±0.19 and 62.75±1.79% inhibitions at 1 mg ml-1 with IC50 of 25, 55 and 195 μg ml-1 respectively. For the AChE inhibition, the Vmax and Km values were noted as 70.08 µg/min and 55.21 μg ml-1 intended for Cb-Cr, 54.38 µg/min and 107.6 μg ml-1 for Cb-Hex, 82.65 µg/min and 51.09 μg ml-1 for Cb-Chf, 72.83 µg/min and 63.05 μg ml-1 for Cb-EtAt, and 64.4 µg/min and 82.27 μg ml-1 for Cb-Aq. likewise, the Vmax and Km values for BChE also displayed effective inhibitory potential of Cb-Cr (63.51 µg/min and 51.82 μg ml-1), Cb-Hex (53.13 µg/min and 47.71 μg ml-1), Cb-Chf (77.37 µg/min and 33.13 μg ml-1), Cb-EtAc (72.28 µg/min and 37.84 μg ml-1), and Cb-Aq (59.18 µg/min and 34.67 μg ml-1), respectively. In conclusion, C. botrys contains bioactive components which can be effective in the curing of Alzheimer's disease (AD) and other stress associated diseases.
Keywords: Chenopodium botrys, Michaelis-Menten
kinetics, Alzheimer's disease, Acetylcholine, Acetylcholinesterase, Butyrylcholinesterase
